00B-4442-E0

Purification and Analysis of Synthetic Oligonucleotides

The Clarity BioSolutions HPLC portfolio includes novel RP-HPLC and IEX-LC columns for purifiying and characterizing synthetic DNA / RNA used in biological research, therapuetic development, and biochemical manufacturing.

• Clarity Oligo-MS™: LC/MS analysis
• Clarity Oligo-RP™: RP-HPLC purification and analysis
• Clarity Oligo-WAX™: IEX-LC purification

RP-HPLC Preparative Purification

Easily isolate target peak from N-1 / N+1 impurities to acheive >90 % purity of most synthetic oligonucleotides eliminating the need for extra purification steps.

• 3 µm, 5 µm, 10 µm particles for seamless scaling
• Cut larger fractions due to baseline resolution
• Increased loadability compared to ion exchange

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Highly Selective RP-HPLC Analysis

Baseline separate DNA or RNA strands with slightly different sequences to resolve difficult synthetic mixtures for easier quantitation.

• Analyze oligos up to 60mer
• High pH (1-12) and temperature stability (80 °C)
• Hydrophobic, dipoloar, pi-pi, hydrogen bonding of sorbent facilitates unique selectivity