by Agilent Technologies
ZORBAX 300StableBond (300SB) HPLC Columns for Biotechnology
ZORBAX 300 StableBond columns are an ideal choice for the reproducible separations of proteins and peptides:
-Wide-pore, 300Å columns are necessary for an efficient separation of proteins and peptides, or other large molecules, in order to allow these analytes to completely access the bonded phase. The 300Stable Bond is an excellent choice.
-300StableBond columns are unmatched in their durability at low pH, such as with the TFA containing mobile phases typically used for protein and peptide separations.
For LC/MS separations at low pH, 300StableBond columns can also be used with formic acid and acetic acid mobile phase modifiers.
Zorbax 300StableBond columns are available in four different bonded phases (C18, C8, C3, and CN) for selectivity and recovery optimization of proteins and polypeptides.
To further increase sample recovery and improve efficiency for difficult proteins, 300StableBond columns can be used up to 80-90 °C. 300SB-C18 and 300SB-C8 columns are an ideal choice for complex protein and protein digest separations.
These columns are available in capillary (0.3, 0.5 mm id) and nano (0.075 and 0.10 mm id) dimensions for reversed-phase LC/MS separations of these protein digests. These capillary and nano columns can be used for either 1-D or 2-D proteomics separations.
| Vendor | Agilent Technologies | Pore size | 300 |
| Brand | ZORBAX 300SB | pH stability from | 1.0 |
| Modifification | Silica/C8 | pH stability to | 8.0 |
| Mode | Reversed-Phase | Surface Area | 45 |
| Particle Size | Carbon Load, % | 1.5 | |
| Length | Endcapped | No | |
| Internal Diameter | Operating Temperature | 90 |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| LabHut | Cronusil-P C8 | Reversed-Phase | Silica/C8 | 3.0 mm | 150 mm | 5.0 μm | 300 Å | 374.10 |
| Princeton Chromatography Inc. | PrincetonSPHER C8-100 | Reversed-Phase | Silica/C8 | 2.0 mm | 75 mm | 3.0 μm | 100 Å | On request |
| Sepax Technologies | Sepax Bio 300 C8 | Reversed-Phase | Silica/C8 | 0.3 mm | 100 mm | 5.0 μm | 300 Å | On request |
| Imtakt | Unison UK-C8 | Reversed-Phase | Silica/C8 | 0.2 mm | 50 mm | 3.0 μm | 130 Å | On request |
| ACE | ACE C8 | Reversed-Phase | Silica/C8 | 3.0 mm | 150 mm | 3.0 μm | 100 Å | On request |
| Bonna-Agela Technologies | Venusil ASB C8 | Reversed-Phase | Silica/C8 | 30.0 mm | 150 mm | 10.0 μm | 150 Å | On request |
| Thermo Fisher Scientific | Acclaim | Reversed-Phase | Silica/C8 | 3.0 mm | 150 mm | 1.2 μm | 120 Å | On request |
| RESTEK | Ultra | Reversed-Phase | Silica/C8 | 3.0 mm | 50 mm | 5.0 μm | 100 Å | On request |
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