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Ascentis C8

by Sigma Aldrich

Ascentis® C8 HPLC Column
3 μm particle size, L × I.D. 10 cm × 1.0 mm          
 
Ascentis®
We have placed heavy emphasis on optimizing Ascentis phases with relation to the three terms of the resolution equation: efficiency, retention and selectivity. However, our strongest emphasis has been on the most powerful term, selectivity. Together, Ascentis bonded phases have a wide range of selectivities. It is likely that one or more Ascentis phase will accomplish any small molecule HPLC separation. Packed in micro- to preparative hardware dimensions, Ascentis products cover all HPLC application areas, including the most sensitive trace-level analyses.

Ascentis C8
High reproducibility, excellent selectivity towards polar compounds

Features and Benefits

  • Superior retention for hydrophobic molecules
  • Excellent peak shape
  • Low bleed LC-MS separations

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.

Application areas:
Aqueous soluble compounds Peptides Small molecules
Specification
Vendor Sigma Aldrich Pore size 100
Brand Ascentis C8 Surface Area 450
Modifification Silica/C8 Carbon Load, % 15.0
Mode Reversed-Phase Endcapped Yes
Particle Size 5.0 Operating Temperature 70
Length 250 pH stability from 2.0
Internal Diameter 2.1 pH stability to 8.0
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