by Sigma Aldrich
Ascentis® C8 HPLC Column
3 μm particle size, L × I.D. 10 cm × 1.0 mm
Ascentis®
We have placed heavy emphasis on optimizing Ascentis phases with relation to the three terms of the resolution equation: efficiency, retention and selectivity. However, our strongest emphasis has been on the most powerful term, selectivity. Together, Ascentis bonded phases have a wide range of selectivities. It is likely that one or more Ascentis phase will accomplish any small molecule HPLC separation. Packed in micro- to preparative hardware dimensions, Ascentis products cover all HPLC application areas, including the most sensitive trace-level analyses.
Ascentis C8
High reproducibility, excellent selectivity towards polar compounds
Features and Benefits
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.
| Vendor | Sigma Aldrich | Pore size | 100 |
| Brand | Ascentis C8 | pH stability from | 2.0 |
| Modifification | Silica/C8 | pH stability to | 8.0 |
| Mode | Reversed-Phase | Surface Area | 450 |
| Particle Size | Carbon Load, % | 15.0 | |
| Length | Endcapped | Yes | |
| Internal Diameter | Operating Temperature | 70 |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Welch Materials | Topsil C8 | Reversed-Phase | Silica/C8 | 3.0 mm | 50 mm | 5.0 μm | 150 Å | On request |
| AcuTech Scientific | C8 | Reversed-Phase | Silica/C8 | 0.32 mm | 500 mm | 3.0 μm | 120 Å | On request |
| Higgins Analytical | HAISIL 100 C8 | Reversed-Phase | Silica/C8 | 0.8 mm | 250 mm | 5.0 μm | 100 Å | On request |
| Bonna-Agela Technologies | Durashell C8 | Reversed-Phase | Silica/C8 | 4.6 mm | 150 mm | 3.0 μm | 100 Å | On request |
| ACE | ACE C8-300 | Reversed-Phase | Silica/C8 | 0.075 mm | 150 mm | 3.0 μm | 300 Å | On request |
| Luknova | Poro C8-300 | Reversed-Phase | Silica/C8 | 3.0 mm | 150 mm | 3.0 μm | 300 Å | On request |
| ES Industries | Chromegabond 100 C8 | Reversed-Phase | Silica/C8 | 3.2 mm | 50 mm | 5.0 μm | 100 Å | On request |
| Agilent Technologies | ZORBAX 300SB | Reversed-Phase | Silica/C8 | 1.0 mm | 150 mm | 3.5 μm | 300 Å | On request |
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