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Ascentis C18

by Sigma Aldrich

Ascentis® C18 HPLC Column
3 μm particle size, L × I.D. 10 cm × 1.0 mm

Ascentis®
We have placed heavy emphasis on optimizing Ascentis phases with relation to the three terms of the resolution equation: efficiency, retention and selectivity. However, our strongest emphasis has been on the most powerful term, selectivity. Together, Ascentis bonded phases have a wide range of selectivities. It is likely that one or more Ascentis phase will accomplish any small molecule HPLC separation. Packed in micro- to preparative hardware dimensions, Ascentis products cover all HPLC application areas, including the most sensitive trace-level analyses.

Ascentis C18
Ascentis C18 is an extremely stable and reliable first choice HPLC column, ideal for LC-MS applications and applicable to a wide range of samples including hydrophobic compounds. Suitable for L1 per USP.

Features and Benefits
• Excellent retention
• Symmetric peak shape
• High reproducibility
• Complete LC-MS compatibility

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis C18 is an extremely stable and reliable first choice HPLC column that gives symmetric peak shape and excellent retention even for difficult compounds.

Application areas:
Hydrophobic compounds Polar compounds
Specification
Vendor Sigma Aldrich Surface Area 450
Brand Ascentis C18 Carbon Load, % 25.0
Mode Reversed-Phase Endcapped Yes
Particle Size 5.0 Operating Temperature 70
Length 250 pH stability from 2.0
Internal Diameter 2.1 pH stability to 8.0
Pore size 100
Recommended applications
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