by Sigma Aldrich
Discovery® BIO Wide Pore C18 HPLC Column
3 μm particle size, L × I.D. 10 cm × 0.32 mm
Discovery BIO Wide Pore C18
HPLC Columns and Capillaries
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
| Vendor | Sigma Aldrich | Internal Diameter | |
| Brand | Discovery BIO Wide Pore C18 | Pore size | 300 |
| Mode | Reversed-Phase | Surface Area | 100 |
| Particle Size | Carbon Load, % | 9.2 | |
| Length | Endcapped | Yes |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| KNAUER | Eurospher II | Reversed-Phase | Silica/C18 | 20.0 mm | 150 mm | 20-45 μm | 100 Å | On request |
| Sepax Technologies | Sepax PolyRP 100 | Reversed-Phase | Polystyrene/ Divinylbenzene/ Phenyl | 21.2 mm | 250 mm | 5.0 μm | 100 Å | On request |
| Luknova | Poro C8-300 | Reversed-Phase | Silica/C8 | 4.6 mm | 100 mm | 5.0 μm | 300 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER PFP-100 | Reversed-Phase | Silica/PFP | 3.0 mm | 150 mm | 5.0 μm | 100 Å | On request |
| KNAUER | Eurosil Bioselect | Reversed-Phase | Silica/C18 | 2.0 mm | 250 mm | 10.0 μm | 300 Å | On request |
| MACHEREY-NAGEL | NUCLEOSHELL RP 18plus | Reversed-Phase | Silica/C18 | 4.0 mm | 50 mm | 2.7 μm | 90 Å | On request |
| Agilent Technologies | Agilent Polaris | Reversed-Phase | Silica/C8 | 2.0 mm | 20 mm | 3.0 μm | 180 Å | On request |
| RESTEK | Ultra | Reversed-Phase | Silica/PFP | 4.6 mm | 100 mm | 5.0 μm | 100 Å | On request |
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Peptides