by Sigma Aldrich
Discovery® BIO Wide Pore C18 HPLC Column
3 μm particle size, L × I.D. 10 cm × 0.32 mm
Discovery BIO Wide Pore C18
HPLC Columns and Capillaries
Peptide maps generated by RP-HPLC provide valuable information about protein structure, stability, and purity. To be effective, the RP-HPLC column must be able to resolve a high percentage of the peptides in the sample. The more peptides, the better the information. Discovery BIO Wide Pore C18 gives unsurpassed RP-HPLC resolution of peptide maps from enzymatic digests. The improvements in silica and bonded-phase chemistry we have incorporated into the Discovery BIO Wide Pore line improve resolution by increasing efficiency and reducing the peak tailing. An added benefit to this is the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.
| Vendor | Sigma Aldrich | Internal Diameter | |
| Brand | Discovery BIO Wide Pore C18 | Pore size | 300 |
| Mode | Reversed-Phase | Surface Area | 100 |
| Particle Size | Carbon Load, % | 9.2 | |
| Length | Endcapped | Yes |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Agilent Technologies | Agilent Polaris | Reversed-Phase | Silica/C8 | 2.0 mm | 20 mm | 5.0 μm | 180 Å | On request |
| Imtakt | Cadenza CD-C18 HT | Reversed-Phase | Silica/C18 | 2.0 mm | 150 mm | 3.0 μm | 120 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C18-60 | Reversed-Phase | Silica/C18 | 3.0 mm | 100 mm | 3.0 μm | 60 Å | On request |
| ES Industries | Chromegabond 100 C8 | Reversed-Phase | Silica/C8 | 2.0 mm | 250 mm | 5.0 μm | 100 Å | On request |
| GRACE | Alltima HP C18 Hi-Load | Reversed-Phase | Silica/C18 | 4.6 mm | 150 mm | 3.0 μm | 100 Å | On request |
| BISCHOFF | ProntoSIL 120-5-Phenyl | Reversed-Phase | Silica/Phenyl | 3.0 mm | 250 mm | 5.0 μm | 120 Å | On request |
| Fortis Technologies | FortisBIO C18 | Reversed-Phase | Silica/C18 | 0.075 mm | 250 mm | 1.7 μm | 300 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER Cyclohexyl-60 | Reversed-Phase | Silica/ Cyclohexyl | 21.2 mm | 250 mm | 5.0 μm | 60 Å | On request |
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Peptides