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Ascentis RP-Amide

by Sigma Aldrich

Ascentis® RP-Amide HPLC Column
3 μm particle size, L × I.D. 10 cm × 1.0 mm
 
Ascentis®
We have placed heavy emphasis on optimizing Ascentis phases with relation to the three terms of the resolution equation: efficiency, retention and selectivity. However, our strongest emphasis has been on the most powerful term, selectivity. Together, Ascentis bonded phases have a wide range of selectivities. It is likely that one or more Ascentis phase will accomplish any small molecule HPLC separation. Packed in micro- to preparative hardware dimensions, Ascentis products cover all HPLC application areas, including the most sensitive trace-level analyses.

Ascentis RP-Amide
Suitable for L60 per USP

Features and Benefits
Excellent retention and peak shape for polar compounds
100% aqueous compatibility
• Ultra low bleed, LC-MS compatible
• Unique selectivity

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Application areas:
Aqueous soluble compounds Basic compounds Peptides Polar compounds Small molecules
Specification
Vendor Sigma Aldrich Pore size 100
Brand Ascentis RP-Amide Surface Area 450
Modifification Silica/RP-Amide Carbon Load, % 19.5
Mode Reversed-Phase Endcapped Yes
Particle Size 5.0 Operating Temperature 70
Length 250 pH stability from 2.0
Internal Diameter 2.1 pH stability to 8.0
Recommended applications
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