by Sigma Aldrich
Ascentis® Phenyl HPLC Column
3 μm particle size, L × I.D. 10 cm × 1.0 mm
Ascentis®
We have placed heavy emphasis on optimizing Ascentis phases with relation to the three terms of the resolution equation: efficiency, retention and selectivity. However, our strongest emphasis has been on the most powerful term, selectivity. Together, Ascentis bonded phases have a wide range of selectivities. It is likely that one or more Ascentis phase will accomplish any small molecule HPLC separation. Packed in micro- to preparative hardware dimensions, Ascentis products cover all HPLC application areas, including the most sensitive trace-level analyses.
Ascentis Phenyl
Suitable for L11 per USP. Exceptional Selectivity for Aromatic Compounds
Features and Benefits
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis Phenyl provides superior separations in reversed-phase mode including 100% aqueous conditions. It may also be used in HILIC/ANP (aqueous normal phase) mode and shows low UV/MS bleed for gradient applications.
| Vendor | Sigma Aldrich | Pore size | 100 |
| Brand | Ascentis Phenyl | pH stability from | 2.0 |
| Modifification | Silica/Phenyl | pH stability to | 8.0 |
| Mode | Reversed-Phase | Surface Area | 450 |
| Particle Size | Carbon Load, % | 19.0 | |
| Length | Endcapped | Yes | |
| Internal Diameter | Operating Temperature | 70 |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Higgins Analytical | CLIPEUS Phenyl | Reversed-Phase | Silica/Phenyl | 2.1 mm | 30 mm | 5.0 μm | 120 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/Phenyl | 4.6 mm | 125 mm | 5.0 μm | 100 Å | On request |
| Sepax Technologies | Sepax GP 120 Phenyl | Reversed-Phase | Silica/Phenyl | 0.5 mm | 150 mm | 1.8 μm | 120 Å | On request |
| Greyhound | GenSphere Phenyl 120 | Reversed-Phase | Silica/Phenyl | 4.0 mm | 150 mm | 3.0 μm | 120 Å | On request |
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/Phenyl | 3.0 mm | 50 mm | 3.0 μm | 175 Å | On request |
| GL Sciences | Inertsil Ph-3 | Reversed-Phase | Silica/Phenyl | 2.1 mm | 100 mm | 3.0 μm | 100 Å | On request |
| Sepax Technologies | Sepax GP 120 Phenyl | Reversed-Phase | Silica/Phenyl | 4.6 mm | 150 mm | 5.0 μm | 120 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER Phenyl-100 | Reversed-Phase | Silica/Phenyl | 4.6 mm | 100 mm | 10.0 μm | 100 Å | On request |
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