by Sigma Aldrich
SUPELCOSIL™ LC-Diol HPLC Column
5 μm particle size, L × I.D. 25 cm × 3.0 mm
SUPELCOSIL™ HPLC Columns
Traditional HPLC columns, similar to Hypersil™
Our SUPELCOSIL silica-based HPLC column line includes over 40 bonded phase chemistries, in a range of particle sizes and column configurations from microbore to preparative scale. It is the original high quality Supelco product line referenced in many USP methods.
In the case of a few SUPLECOSIL phases, we have recommended alternatives based on more advanced column technology.
LC-Diol
Suitable for L20 per USP
LC-Diol columns can be used to separate proteins by gel filtration chromatography; when operated with an aqueous buffer, the diol phase shields the silica surface from interacting with proteins. The separation of steroids and sterols is commonly done using a diol column under normal phase conditions.
| Vendor | Sigma Aldrich | Pore size | 120 |
| Brand | SUPELCOSIL LC-Diol | Surface Area | 170 |
| Modifification | Silica/Diol | Carbon Load, % | 3.5 |
| Mode | Reversed-Phase | Endcapped | No |
| Particle Size | Operating Temperature | 70 | |
| Length | pH stability from | 2.0 | |
| Internal Diameter | pH stability to | 7.5 |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| KNAUER | Eurospher II | Reversed-Phase | Silica/Diol | 8.0 mm | 120 mm | 5.0 μm | 100 Å | On request |
| ES Industries | Chromegabond 60 D/RP | Reversed-Phase | Silica/Diol | 4.6 mm | 100 mm | 5.0 μm | 60 Å | On request |
| ES Industries | Chromegabond 4000 D/RP | Reversed-Phase | Silica/Diol | 2.0 mm | 250 mm | 5.0 μm | 4000 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/Diol | 4.6 mm | 250 mm | 10.0 μm | 100 Å | On request |
| ES Industries | Chromegabond 100 D/RP | Reversed-Phase | Silica/Diol | 4.0 mm | 100 mm | 3.0 μm | 100 Å | On request |
| ES Industries | Chromegabond 1000 D/RP | Reversed-Phase | Silica/Diol | 4.0 mm | 100 mm | 5.0 μm | 1000 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/Diol | 4.6 mm | 250 mm | 5.0 μm | 100 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/Diol | 62.0 mm | 30 mm | 3.0 μm | 100 Å | On request |
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