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Catalog Search results 56400AST

56400AST

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apHera NH2

by Sigma Aldrich

apHera™ NH2 HPLC Column
5 μm particle size, L × I.D. 15 cm × 2.0 mm

apHera™ Amino (NH2) Columns
apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera™ Columns

High efficiency for carbohydrate analysis
Ideal for basic conditions/amphoteric detection
High selectivity mono- to oligosaccharides
pH range 2-13
300 angstrom pore size
Stable, predictable retention
Long column life

Application areas:

Monosaccharides/disaccharides Oligosaccharides

Specification

Vendor	Sigma Aldrich	Particle Size
Brand	apHera NH2	Length
Modifification	Silica/Amino	Internal Diameter
Mode	Reversed-Phase

Similar columns Columns with same mode & modification — across all brands

Vendor	Brand	Mode	Modification	Int. diam.	Length	Particles	Pores	Price
Orochem	Matrix NH2	Reversed-Phase	Silica/Amino	3.0 mm	50 mm	5.0 μm	100 Å	On request
PerkinElmer	Brownlee Analytical Amino	Reversed-Phase	Silica/Amino	4.6 mm	50 mm	3.0 μm	110 Å	On request
Merck	Purospher® STAR	Reversed-Phase	Silica/Amino	4.6 mm	150 mm	5.0 μm	120 Å	On request
MACHEREY-NAGEL	NUCLEODUR NH2-RP	Reversed-Phase	Silica/Amino	2.0 mm	125 mm	5.0 μm	110 Å	279.00
KNAUER	Eurospher II	Reversed-Phase	Silica/Amino	4.0 mm	100 mm	10.0 μm	100 Å	On request
Merck	LiChrospher®	Reversed-Phase	Silica/Amino	4.0 mm	250 mm	10.0 μm	100 Å	On request
ES Industries	Chromegabond 100 A/RP	Reversed-Phase	Silica/Amino	4.0 mm	50 mm	5.0 μm	100 Å	On request
KNAUER	Eurospher II	Reversed-Phase	Silica/Amino	20.0 mm	250 mm	15.0 μm	100 Å	On request

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