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56403AST

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56403AST
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apHera NH2

by Sigma Aldrich

apHera™ NH2 HPLC Column
5 μm particle size, L × I.D. 15 cm × 2.0 mm

apHera™ Amino (NH2) Columns
apHera™ amino columns are based on covalently bonded polyamine specifically optimized for the separation of mono- and oligosaccharides. The elution order mono-, di-, tri-saccharide shows increased eluton volume with increased acetonitrile concentration and complete stability for both acidic and alkaline eluates. The small, robust PVA copolymer bead provides mechanical and chemical strength as well as high column efficiency. Conventional camion columns based on silica do not show long column life, perhaps due to hydrolysis of silica particle by the basic amino group. Since Supelco uses a strong alkaline compatible polymer, these problems are eliminated. Stable retention time and long column life are also characteristic of the column.

Features of Amino apHera™ Columns

  • High efficiency for carbohydrate analysis
  • Ideal for basic conditions/amphoteric detection
  • High selectivity mono- to oligosaccharides
  • pH range 2-13
  • 300 angstrom pore size
  • Stable, predictable retention
  • Long column life
Application areas:
Monosaccharides/disaccharides Oligosaccharides
Specification
Vendor Sigma Aldrich Particle Size
Brand apHera NH2 Length
Modifification Silica/Amino Internal Diameter
Mode Reversed-Phase
Recommended applications
Similar columns Columns with same mode & modification — across all brands
VendorBrandModeModification Int. diam.LengthParticlesPoresPrice
KNAUER Eurospher II Reversed-Phase Silica/Amino 4.6 mm 250 mm 3.0 μm 100 Å On request
ES Industries Chromegabond 300 A/RP Reversed-Phase Silica/Amino 3.2 mm 250 mm 5.0 μm 300 Å On request
Orochem Reliasil NH2 90 Reversed-Phase Silica/Amino 3.0 mm 150 mm 3.0 μm 90 Å On request
Orochem Reliasil NH2 90 Reversed-Phase Silica/Amino 21.2 mm 100 mm 5.0 μm 90 Å On request
KNAUER Eurospher II Reversed-Phase Silica/Amino 2.0 mm 150 mm 5.0 μm 100 Å On request
KNAUER Eurospher II Reversed-Phase Silica/Amino 3.0 mm 150 mm 3.0 μm 100 Å On request
RSTech OptimaPak NH2 Reversed-Phase Silica/Amino 2.1 mm 100 mm 5.0 μm — On request
Orochem Reliasil NH2 90 Reversed-Phase Silica/Amino 4.6 mm 30 mm 5.0 μm 90 Å On request
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