by Sigma Aldrich
Ascentis® Si HPLC Column
3 μm particle size, L × I.D. 10 cm × 1.0 mm
Ascentis®
We have placed heavy emphasis on optimizing Ascentis phases with relation to the three terms of the resolution equation: efficiency, retention and selectivity. However, our strongest emphasis has been on the most powerful term, selectivity. Together, Ascentis bonded phases have a wide range of selectivities. It is likely that one or more Ascentis phase will accomplish any small molecule HPLC separation. Packed in micro- to preparative hardware dimensions, Ascentis products cover all HPLC application areas, including the most sensitive trace-level analyses.
Ascentis Si
Suitable for L3 per USP
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis Si is a high loading capacity silica with excellent peak shape. The Ascentis Si performs in both normal-phase and HILIC/ANP (aqueous normal phase) mode.
| Vendor | Sigma Aldrich | Pore size | 100 |
| Brand | Ascentis Si | Surface Area | 450 |
| Modifification | Silica | Endcapped | No |
| Mode | Normal-Phase | Operating Temperature | 70 |
| Particle Size | pH stability from | 2.0 | |
| Length | pH stability to | 6.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| SiliCYCLE | SiliaChrom dt Si | Normal-Phase | Silica | 3.0 mm | 30 mm | 3.0 μm | 100 Å | On request |
| RESTEK | Pinnacle DB | Normal-Phase | Silica | 4.6 mm | 30 mm | 3.0 μm | 140 Å | On request |
| Thermo Fisher Scientific | Syncronis | Normal-Phase | Silica | 4.6 mm | 250 mm | 5.0 μm | 100 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER SILICA-100 | Normal-Phase | Silica | 3.0 mm | 150 mm | 3.0 μm | 100 Å | On request |
| ES Industries | Chromegasphere SI 4000 (Spherical) | Normal-Phase | Silica | 2.0 mm | 250 mm | 5.0 μm | 4000 Å | On request |
| Sepax Technologies | Sepax HP 120 Silica | Normal-Phase | Silica | 1.0 mm | 50 mm | 3.0 μm | 120 Å | On request |
| ES Industries | Chromegasphere SI 120 (Spherical) | Normal-Phase | Silica | 3.2 mm | 300 mm | 10.0 μm | 120 Å | On request |
| BISCHOFF | ProntoSIL 300-5-Si | Normal-Phase | Silica | 4.0 mm | 125 mm | 5.0 μm | 300 Å | On request |
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