by Sigma Aldrich
Ascentis® Express F5 HPLC Column
2.7 μm particle size, L × I.D. 10 cm × 2.1 mm
Ascentis® Express F5 HPLC Column
The pentafluorophenylpropyl (PFP) stationary phase of Ascentis™ Express F5 provides a stable, reversed-phase packing with electron-deficient phenyl rings due to the presence of electronegative fluorines. In addition to forming p-p and mildly steric interactions, F5 phases also retain compounds by polar interactions. As a result of having both polar and non-polar character, F5 phases can show dual-mode retention behavior, sometimes producing a “U-shaped” retention as a function of acetonitrile content of the mobile phase, with retention increasing at both low and high concentrations of ACN (reversed-phase and HILIC retention modes). Ascentis Express F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.
| Vendor | Sigma Aldrich | Internal Diameter | |
| Brand | Ascentis Express F5 | Pore size | 90 |
| Modifification | Fused-Core silica | Endcapped | Yes |
| Mode | Reversed-Phase | Operating Temperature | 100 |
| Particle Size | pH stability from | 1.0 | |
| Length | pH stability to | 9.0 |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Sigma Aldrich | Ascentis Express F5 | Reversed-Phase | Fused-Core silica | 2.1 mm | 50 mm | 2.7 μm | 90 Å | On request |
| Sigma Aldrich | Ascentis Express F5 | Reversed-Phase | Fused-Core silica | 3.0 mm | 150 mm | 2.7 μm | 90 Å | On request |
| Sigma Aldrich | Ascentis Express F5 | Reversed-Phase | Fused-Core silica | 2.1 mm | 20 mm | 2.7 μm | 90 Å | On request |
| Sigma Aldrich | Ascentis Express F5 | Reversed-Phase | Fused-Core silica | 3.0 mm | 75 mm | 2.7 μm | 90 Å | On request |
| Sigma Aldrich | Ascentis Express F5 | Reversed-Phase | Fused-Core silica | 3.0 mm | 50 mm | 2.7 μm | 90 Å | On request |
| Sigma Aldrich | Ascentis Express F5 | Reversed-Phase | Fused-Core silica | 4.6 mm | 75 mm | 2.7 μm | 90 Å | On request |
| Sigma Aldrich | Ascentis Express F5 | Reversed-Phase | Fused-Core silica | 3.0 mm | 30 mm | 2.7 μm | 90 Å | On request |
| Sigma Aldrich | Ascentis Express F5 | Reversed-Phase | Fused-Core silica | 2.1 mm | 150 mm | 2.7 μm | 90 Å | On request |
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Drugs of Abuse, Pharmaceuticals