by Sigma Aldrich
SUPELCOSIL™ LC-NH2-NP HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
SUPELCOSIL™ HPLC Columns
Traditional HPLC columns, similar to Hypersil™
Our SUPELCOSIL silica-based HPLC column line includes over 40 bonded phase chemistries, in a range of particle sizes and column configurations from microbore to preparative scale. It is the original high quality Supelco product line referenced in many USP methods.
In the case of a few SUPLECOSIL phases, we have recommended alternatives based on more advanced column technology.
SUPELCOSIL LC-NH2-NP columns:
Features and Benefits
An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K.
These columns should be used with non-aqueous mobile phases only.
| Vendor | Sigma Aldrich | Pore size | 120 |
| Brand | SUPELCOSIL LC-NH2-NP | Surface Area | 170 |
| Modifification | Silica/ Aminopropyl | Carbon Load, % | 3.0 |
| Mode | Normal-Phase+CX | Endcapped | Yes |
| Particle Size | 5.0 | Operating Temperature | 70 |
| Length | 250 | pH stability from | 2.0 |
| Internal Diameter | 4.6 | pH stability to | 7.5 |
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