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59132
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SUPELCOSIL LC-NH2-NP

by Sigma Aldrich

SUPELCOSIL™ LC-NH2-NP HPLC Column
5 μm particle size, L × I.D. 25 cm × 4.6 mm
 
SUPELCOSIL™ HPLC Columns
Traditional HPLC columns, similar to Hypersil™
Our SUPELCOSIL silica-based HPLC column line includes over 40 bonded phase chemistries, in a range of particle sizes and column configurations from microbore to preparative scale. It is the original high quality Supelco product line referenced in many USP methods.
In the case of a few SUPLECOSIL phases, we have recommended alternatives based on more advanced column technology.

SUPELCOSIL LC-NH2-NP columns:

  • show stable retention in normal phase separations
  • are less sensitive to small or varying amounts of water in the mobile phase, relative to unmodified silica
  • provide excellent separations of fat-soluble vitamins

Features and Benefits

  • show stable retention in normal-phase separations
  • are less sensitive to small or varying amounts of water in mobile phases, relative to unmodified silica
  • provide excellent separations of fat-soluble vitamins

An amino phase dedicated to normal-phase chromatography. By employing special bonding technology, and avoiding water in manufacturing and testing the column, we have dramatically reduced the retention variation that is characteristic of normal-phase chromatography. Normal-phase chromatography is especially useful when the analytes are not water soluble -- for example, the fat-soluble vitamins A, D, E, and K.
These columns should be used with non-aqueous mobile phases only.

Application areas:
Vitamins
Specification
Vendor Sigma Aldrich Pore size 120
Brand SUPELCOSIL LC-NH2-NP Surface Area 170
Modifification Silica/ Aminopropyl Carbon Load, % 3.0
Mode Normal-Phase+CX Endcapped Yes
Particle Size 5.0 Operating Temperature 70
Length 250 pH stability from 2.0
Internal Diameter 4.6 pH stability to 7.5
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