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56308AST

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apHera C4

by Sigma Aldrich

apHera™ C4 HPLC Column
5 μm particle size, L × I.D. 15 cm × 4.6 mm

apHera™ C4 Reversed-phase Columns
apHera™ reversed columns were devoloped specifically to provide the superior advantages of both silica and polystyrene columns, without the disadvantages of either. This was accomplished using a vinyl alcohol copolymer base that keeps the surface wetted even with high carbon loads. The porous structure has an average pore diameter large enough to produce ideal results for small analytes, peptides and small proteins. These column equal silica based columns in separation efficiency with organic solvents but provide efficiency with buffered alkaline solutions not possible on silica. One of the most significant features in logicial elution order of alkylated bases where retention increases proportionately with increasing chain length.

Features of apHera™ Reversed Phase Columns

Stable vinyl copolymer base
Stable pH range 2-12
300 angstrom pore size
Stable in all organic solvents
Expanded applications: peptides, proteins
Efficient separation of any basic substance
Amenability to washing and alkaline solutions

Application areas:

Peptides Proteins Small molecules

Specification

Vendor	Sigma Aldrich	Particle Size
Brand	apHera C4	Length
Modifification	Silica/C4	Internal Diameter
Mode	Reversed-Phase

Similar columns Columns with same mode & modification — across all brands

Vendor	Brand	Mode	Modification	Int. diam.	Length	Particles	Pores	Price
Princeton Chromatography Inc.	PrincetonSPHER C4-100	Reversed-Phase	Silica/C4	4.0 mm	50 mm	3.0 μm	100 Å	On request
Nacalai	COSMOSIL 5C4-MS	Reversed-Phase	Silica/C4	6.0 mm	250 mm	5.0 μm	120 Å	On request
Biotage	Resolux 300 C4	Reversed-Phase	Silica/C4	4.6 mm	250 mm	4.5 μm	300 Å	On request
Higgins Analytical	PROTO 300 C4	Reversed-Phase	Silica/C4	0.8 mm	100 mm	5.0 μm	300 Å	On request
Higgins Analytical	PROTO 200 C4	Reversed-Phase	Silica/C4	0.8 mm	100 mm	5.0 μm	200 Å	On request
Thermo Fisher Scientific	Hypesil GOLD	Reversed-Phase	Silica/C4	4.6 mm	30 mm	3.0 μm	175 Å	On request
KNAUER	Eurospher II	Reversed-Phase	Silica/C4	3.0 mm	50 mm	3.0 μm	100 Å	On request
Luknova	Poro C4-300	Reversed-Phase	Silica/C4	10.0 mm	250 mm	5.0 μm	300 Å	On request

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