by Sigma Aldrich
Ascentis® C8 HPLC Column
3 μm particle size, L × I.D. 10 cm × 1.0 mm
Ascentis®
We have placed heavy emphasis on optimizing Ascentis phases with relation to the three terms of the resolution equation: efficiency, retention and selectivity. However, our strongest emphasis has been on the most powerful term, selectivity. Together, Ascentis bonded phases have a wide range of selectivities. It is likely that one or more Ascentis phase will accomplish any small molecule HPLC separation. Packed in micro- to preparative hardware dimensions, Ascentis products cover all HPLC application areas, including the most sensitive trace-level analyses.
Ascentis C8
High reproducibility, excellent selectivity towards polar compounds
Features and Benefits
The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.
The Ascentis C8 is a highly reproducible column with excellent selectivity towards polar compounds. The phase excells in highly aqueous mobile phases as well as high organic mobile phases.
| Vendor | Sigma Aldrich | pH stability from | 2.0 |
| Brand | Ascentis C8 | pH stability to | 8.0 |
| Modifification | Silica/C8 | Surface Area | 450 |
| Mode | Reversed-Phase | Carbon Load, % | 15.0 |
| Particle Size | Endcapped | Yes | |
| Length | Operating Temperature | 70 | |
| Internal Diameter | Product usage | Aqueous soluble compounds | |
| Pore size | 100 |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Agilent Technologies | Agilent Polaris | Reversed-Phase | Silica/C8 | 2.0 mm | 150 mm | 3.0 μm | 180 Å | On request |
| Vertical Chromatography | VertiSep AQS C8 | Reversed-Phase | Silica/C8 | 2.1 mm | 150 mm | 3.0 μm | 120 Å | On request |
| Teknokroma | Europa Protein 300 C8 | Reversed-Phase | Silica/C8 | 2.1 mm | 200 mm | 5.0 μm | 300 Å | On request |
| ACE | ACE C8-300 | Reversed-Phase | Silica/C8 | 0.3 mm | 100 mm | 5.0 μm | 300 Å | On request |
| GL Sciences | Inertsil C8-3 | Reversed-Phase | Silica/C8 | 1.5 mm | 33 mm | 5.0 μm | 100 Å | On request |
| Merck | Purospher® STAR | Reversed-Phase | Silica/C8 | 4.6 mm | 250 mm | 5.0 μm | 120 Å | On request |
| Agilent Technologies | Agilent Polaris | Reversed-Phase | Silica/C8 | 4.0 mm | 250 mm | 5.0 μm | 180 Å | On request |
| Sepax Technologies | Sepax Bio 300 C8 | Reversed-Phase | Silica/C8 | 0.5 mm | 100 mm | 5.0 μm | 300 Å | On request |
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