by KNAUER
Bluespher columns make method transfer from HPLC to UHPLC easy. They were developed to feature very similar characteristics and selectivities to the Eurospher I and II families. Take HPLC to the next level with ultra high performance Bluespher columns.
Bluespher columns are packed with ultra pure silica stationary phase to provide excellent separation performance and are well-suited for either routine analysis or ambitious chromatography in high speed mode where resolution, sensitivity and sample throughput are critical. These columns are your first choice for high-throughput-screening, quality control, and method development.
Take advantage of the comparable selectivities of Eurospher, Eurospher II, and Bluespher columns to easily scale down your existing analytical method to UHPLC as well as to scale it up to semi-prep or even preparative LC.
Finding the appropriate column for a particular application can be a challenging task. With a range of bonded phases offering different selectivity, the Bluespher family includes columns to meet most separation needs. The chart below will help you to choose the best Bluespher column for a particular application.
|
Modification |
Application areas |
Separation mechanism |
|
C18 |
for acidic, basic and neutral analytes in reversed phase mode (sulphonamides; anabolic steroids; anti-psychotics; beta blocker; Sudan dyes; phenols, preservatives etc.) |
hydrophobic interaction |
| Vendor | KNAUER | Internal Diameter | |
| Brand | Bluespher | Pore size | 100 |
| Modifification | Silica | Surface Area | 320 |
| Mode | Normal-Phase | pH stability from | 2.0 |
| Particle Size | pH stability to | 8.0 | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| ES Industries | Chromegasphere SI 120 (Spherical) | Normal-Phase | Silica | 4.0 mm | 150 mm | 10.0 μm | 120 Å | On request |
| Agilent Technologies | Agilent Polaris | Normal-Phase | Silica | 2.0 mm | 150 mm | 5.0 μm | 180 Å | On request |
| Sigma Aldrich | Kromasil Silica | Normal-Phase | Silica | 4.6 mm | 250 mm | 16.0 μm | 100 Å | On request |
| BISCHOFF | ProntoSIL 120-3-Si | Normal-Phase | Silica | 2.0 mm | 250 mm | 3.0 μm | 120 Å | On request |
| Thermo Fisher Scientific | Syncronis | Normal-Phase | Silica | 4.6 mm | 250 mm | 5.0 μm | 100 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER SILICA-60 | Normal-Phase | Silica | 10.0 mm | 50 mm | 5.0 μm | 60 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER SILICA-300 | Normal-Phase | Silica | 50.0 mm | 150 mm | 10.0 μm | 300 Å | On request |
| Axxiom Chromatography | Axxiom Si | Normal-Phase | Silica | 10.0 mm | 150 mm | 5.0 μm | — | On request |
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