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10BE000BSF
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Bluespher

by KNAUER

Bluespher: take HPLC to the next level with ultra high performance Bluespher columns                              

Vertex Plus Column Bluespher C18                               

Bluespher columns make method transfer from HPLC to UHPLC easy. They were developed to feature very similar characteristics and selectivities to the Eurospher I and II families. Take HPLC to the next level with ultra high performance Bluespher columns.

Bluespher columns are packed with ultra pure silica stationary phase to provide excellent separation performance and are well-suited for either routine analysis or ambitious chromatography in high speed mode where resolution, sensitivity and sample throughput are critical. These columns are your first choice for high-throughput-screening, quality control, and method development.

Scale up and scale down

Take advantage of the comparable selectivities of Eurospher, Eurospher II, and Bluespher columns to easily scale down your existing analytical method to UHPLC as well as to scale it up to semi-prep or even preparative LC.

Bluespher applications

Finding the appropriate column for a particular application can be a challenging task. With a range of bonded phases offering different selectivity, the Bluespher family includes columns to meet most separation needs. The chart below will help you to choose the best Bluespher column for a particular application.

Modification

Application areas

Separation mechanism

C18

for acidic, basic and neutral analytes in reversed phase mode (sulphonamides; anabolic steroids; anti-psychotics; beta blocker; Sudan dyes; phenols, preservatives etc.)

hydrophobic interaction

Specification
Vendor KNAUER Internal Diameter
Brand Bluespher Pore size 100
Modifification Silica Surface Area 320
Mode Normal-Phase pH stability from 2.0
Particle Size pH stability to 8.0
Length
Similar columns Columns with same mode & modification — across all brands
VendorBrandModeModification Int. diam.LengthParticlesPoresPrice
ES Industries Chromegasphere SI 120 (Spherical) Normal-Phase Silica 4.0 mm 150 mm 10.0 μm 120 Å On request
Agilent Technologies Agilent Polaris Normal-Phase Silica 2.0 mm 150 mm 5.0 μm 180 Å On request
Sigma Aldrich Kromasil Silica Normal-Phase Silica 4.6 mm 250 mm 16.0 μm 100 Å On request
BISCHOFF ProntoSIL 120-3-Si Normal-Phase Silica 2.0 mm 250 mm 3.0 μm 120 Å On request
Thermo Fisher Scientific Syncronis Normal-Phase Silica 4.6 mm 250 mm 5.0 μm 100 Å On request
Princeton Chromatography Inc. PrincetonSPHER SILICA-60 Normal-Phase Silica 10.0 mm 50 mm 5.0 μm 60 Å On request
Princeton Chromatography Inc. PrincetonSPHER SILICA-300 Normal-Phase Silica 50.0 mm 150 mm 10.0 μm 300 Å On request
Axxiom Chromatography Axxiom Si Normal-Phase Silica 10.0 mm 150 mm 5.0 μm On request
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