by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C4 | Pore size | 200 |
| Modifification | Silica/C4 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 4.5 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Princeton Chromatography Inc. | PrincetonSPHER C4-60 | Reversed-Phase | Silica/C4 | 50.0 mm | 150 mm | 5.0 μm | 60 Å | On request |
| Welch Materials | Ultisil XB-C4 | Reversed-Phase | Silica/C4 | 4.0 mm | 50 mm | 5.0 μm | 300 Å | On request |
| RESTEK | Viva | Reversed-Phase | Silica/C4 | 2.1 mm | 250 mm | 5.0 μm | 300 Å | On request |
| GL Sciences | Inertsil WP300 C4 | Reversed-Phase | Silica/C4 | 4.0 mm | 30 mm | 5.0 μm | 300 Å | On request |
| ACE | ACE C4 | Reversed-Phase | Silica/C4 | 1.0 mm | 20 mm | 5.0 μm | 100 Å | On request |
| GRACE | Vydac TP C4 | Reversed-Phase | Silica/C4 | 22.0 mm | 150 mm | 10.0 μm | 300 Å | On request |
| MACHEREY-NAGEL | NUCLEODUR C4 EC | Reversed-Phase | Silica/C4 | 2.0 mm | 150 mm | 5.0 μm | 300 Å | On request |
| Dikma Technologies Inc. | Bio-Bond C4 | Reversed-Phase | Silica/C4 | 4.6 mm | 50 mm | 3.0 μm | 300 Å | 373.00 |
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