by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C18 | Pore size | 200 |
| Modifification | Silica/C18 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 18.0 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Aapptec | Spirit Peptide | Reversed-Phase | Silica/C18 | 4.0 mm | 200 mm | 5.0 μm | 120 Å | On request |
| Orochem | Prestige C18 | Reversed-Phase | Silica/C18 | 1.0 mm | 50 mm | 3.0 μm | 100 Å | On request |
| Thermo Fisher Scientific | BetaMax | Reversed-Phase | Silica/C18 | 2.1 mm | 50 mm | 5.0 μm | 60 Å | On request |
| Teknokroma | Europa Protein 300 C18 | Reversed-Phase | Silica/C18 | 3.0 mm | 250 mm | 5.0 μm | 300 Å | On request |
| BISCHOFF | ProntoSIL Spheribond ODS2 | Reversed-Phase | Silica/C18 | 4.0 mm | 150 mm | 3.0 μm | 80 Å | On request |
| Sepax Technologies | Sepax GP 120 C18 | Reversed-Phase | Silica/C18 | 0.075 mm | 150 mm | 3.0 μm | 120 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/C18 | 8.0 mm | 300 mm | 10.0 μm | 100 Å | On request |
| Fortis Technologies | Fortis C18 | Reversed-Phase | Silica/C18 | 3.0 mm | 30 mm | 3.0 μm | — | On request |
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