by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C18 | Pore size | 200 |
| Modifification | Silica/C18 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 18.0 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| SGE Analytical Science | ProteCol C18 G (PEEK Coated) | Reversed-Phase | Silica/C18 | 4.6 mm | 150 mm | 3.0 μm | 200 Å | On request |
| AcuTech Scientific | C18 | Reversed-Phase | Silica/C18 | 3.0 mm | 50 mm | 3.0 μm | 120 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER OMNI C18-60 | Reversed-Phase | Silica/C18 | 2.0 mm | 75 mm | 5.0 μm | 60 Å | On request |
| SiliCYCLE | SiliaChrom XT C18 | Reversed-Phase | Silica/C18 | 20.0 mm | 100 mm | 5.0 μm | 150 Å | On request |
| Thermo Fisher Scientific | BetaSil | Reversed-Phase | Silica/C18 | 3.0 mm | 50 mm | 5.0 μm | 100 Å | On request |
| WATERS | Spherisorb ODS2 | Reversed-Phase | Silica/C18 | 3.0 mm | 250 mm | 5.0 μm | 80 Å | On request |
| ACE | ACE C18-HL | Reversed-Phase | Silica/C18 | 4.6 mm | 125 mm | 5.0 μm | 90 Å | On request |
| ACE | ACE UltraCore 5 Super C18 | Reversed-Phase | Silica/C18 | 3.0 mm | 75 mm | 5.0 μm | 95 Å | On request |
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