by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C4 | Pore size | 200 |
| Modifification | Silica/C4 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 4.5 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| CHEMINDIA | CHEMSIL-BUTYL-C4 | Reversed-Phase | Silica/C4 | 4.0 mm | 75 mm | 3.0 μm | 100 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/C4 | 20.0 mm | 250 mm | 15.0 μm | 100 Å | On request |
| GL Sciences | Inertsil C4 | Reversed-Phase | Silica/C4 | 4.0 mm | 250 mm | 5.0 μm | 150 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C4-300 | Reversed-Phase | Silica/C4 | 4.0 mm | 75 mm | 10.0 μm | 300 Å | On request |
| KYA Technologies Corporation | HiQ Sil C4P-300 | Reversed-Phase | Silica/C4 | 4.6 mm | 150 mm | 5.0 μm | 300 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C4-60 | Reversed-Phase | Silica/C4 | 2.0 mm | 100 mm | 3.0 μm | 60 Å | On request |
| Nacalai | COSMOSIL 5C4-MS | Reversed-Phase | Silica/C4 | 10.0 mm | 250 mm | 5.0 μm | 120 Å | On request |
| RESTEK | Ultra | Reversed-Phase | Silica/C4 | 4.6 mm | 150 mm | 3.0 μm | 100 Å | On request |
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