by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C18 | Pore size | 200 |
| Modifification | Silica/C18 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 18.0 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| SiliCYCLE | SiliaChrom XT Fidelity C18 | Reversed-Phase | Silica/C18 | 10.0 mm | 150 mm | 10.0 μm | 150 Å | On request |
| Agilent Technologies | Poroshell 120 | Reversed-Phase | Silica/C18 | 3.0 mm | 50 mm | 2.7 μm | 120 Å | On request |
| Agilent Technologies | Agilent Polaris | Reversed-Phase | Silica/C18 | 4.0 mm | 75 mm | 3.0 μm | 180 Å | On request |
| Merck | Chromolith® | Reversed-Phase | Silica/C18 | 0.2 mm | 150 mm | Monolithic μm | 130 Å | On request |
| Agilent Technologies | Agilent Pursuit | Reversed-Phase | Silica/C18 | 4.0 mm | 250 mm | 5.0 μm | 180 Å | On request |
| Agilent Technologies | ZORBAX Stablebond | Reversed-Phase | Silica/C18 | 2.0 mm | 50 mm | 1.8 μm | 80 Å | On request |
| Fortis Technologies | UniverSil C18 | Reversed-Phase | Silica/C18 | 4.0 mm | 125 mm | 3.0 μm | 140 Å | On request |
| Thermo Fisher Scientific | BioBasic | Reversed-Phase | Silica/C18 | 21.0 mm | 150 mm | 5.0 μm | 300 Å | On request |
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