by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C18 | Pore size | 200 |
| Modifification | Silica/C18 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 18.0 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| GRACE | VisionHT C18 Classic | Reversed-Phase | Silica/C18 | 2.1 mm | 50 mm | 3.0 μm | 100 Å | On request |
| Imtakt | Intrada WP-RP | Reversed-Phase | Silica/C18 | 3.0 mm | 50 mm | 3.0 μm | 300 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C18-100 | Reversed-Phase | Silica/C18 | 2.0 mm | 150 mm | 5.0 μm | 100 Å | On request |
| Princeton Chromatography Inc. | Ultima C18-100 | Reversed-Phase | Silica/C18 | 4.0 mm | 75 mm | 5.0 μm | 100 Å | On request |
| Fortis Technologies | UniverSil HS C18 | Reversed-Phase | Silica/C18 | 4.0 mm | 125 mm | 5.0 μm | 100 Å | On request |
| BISCHOFF | ProntoSIL 120-3-C18 ace-EPS PEEK | Reversed-Phase | Silica/C18 | 4.0 mm | 200 mm | 3.0 μm | 120 Å | On request |
| Sepax Technologies | Sepax BR 120 C18 | Reversed-Phase | Silica/C18 | 0.75 mm | 50 mm | 3.0 μm | 120 Å | On request |
| Dikma Technologies Inc. | Spursil C18-EP | Reversed-Phase | Silica/C18 | 4.6 mm | 100 mm | 5.0 μm | 100 Å | 527.00 |
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