by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C4 | Pore size | 200 |
| Modifification | Silica/C4 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 4.5 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Higgins Analytical | PROTO 300 C4 | Reversed-Phase | Silica/C4 | 1.0 mm | 50 mm | 5.0 μm | 300 Å | On request |
| WATERS | Symmetry 300 C4 | Reversed-Phase | Silica/C4 | 4.6 mm | 150 mm | 5.0 μm | 300 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C4-60 | Reversed-Phase | Silica/C4 | 2.0 mm | 150 mm | 3.0 μm | 60 Å | On request |
| ACE | ACE C4-300 | Reversed-Phase | Silica/C4 | 21.2 mm | 150 mm | 10.0 μm | 300 Å | On request |
| Thermo Fisher Scientific | Hypurity | Reversed-Phase | Silica/C4 | 4.0 mm | 100 mm | 5.0 μm | 190 Å | On request |
| KNAUER | Eurosil Bioselect | Reversed-Phase | Silica/C4 | 4.0 mm | 250 mm | 3.0 μm | 300 Å | On request |
| RESTEK | Ultra | Reversed-Phase | Silica/C4 | 2.1 mm | 100 mm | 5.0 μm | 100 Å | On request |
| Chromenta | Chromenta EP C4 | Reversed-Phase | Silica/C4 | 3.0 mm | 150 mm | 3.0 μm | 120 Å | On request |
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