by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C18 | Pore size | 200 |
| Modifification | Silica/C18 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 18.0 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/C18 | 0.075 mm | 100 mm | 1.9 μm | 175 Å | On request |
| WATERS | Atlantis C18 | Reversed-Phase | Silica/C18 | 4.6 mm | 30 mm | 5.0 μm | 100 Å | On request |
| Higgins Analytical | HAISIL HL C18 | Reversed-Phase | Silica/C18 | 0.3 mm | 50 mm | 5.0 μm | 100 Å | On request |
| Thermo Fisher Scientific | Aquasil | Reversed-Phase | Silica/C18 | 2.1 mm | 100 mm | 5.0 μm | 100 Å | On request |
| Fortis Technologies | UniverSil C18 | Reversed-Phase | Silica/C18 | 3.0 mm | 50 mm | 3.0 μm | 140 Å | On request |
| Greyhound | Spheripak ODS2 80 | Reversed-Phase | Silica/C18 | 4.6 mm | 250 mm | 10.0 μm | 80 Å | On request |
| Aapptec | Spirit Peptide | Reversed-Phase | Silica/C18 | 4.0 mm | 40 mm | 5.0 μm | 120 Å | On request |
| AcuTech Scientific | C18 | Reversed-Phase | Silica/C18 | 0.075 mm | 350 mm | 3.0 μm | 120 Å | On request |
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