by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C18 | Pore size | 200 |
| Modifification | Silica/C18 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 18.0 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| ES Industries | Chromegabond 60 MC-18 | Reversed-Phase | Silica/C18 | 3.2 mm | 50 mm | 3.0 μm | 60 Å | On request |
| PerkinElmer | Brownlee Analytical DB Aqueous C18 | Reversed-Phase | Silica/C18 | 4.6 mm | 50 mm | 5.0 μm | — | On request |
| Imtakt | Cadenza CD-C18 HT | Reversed-Phase | Silica/C18 | 4.6 mm | 100 mm | 3.0 μm | 120 Å | On request |
| ES Industries | Chromegabond 100 ProTec C18 | Reversed-Phase | Silica/C18 | 4.6 mm | 100 mm | 5.0 μm | 100 Å | On request |
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/C18 | 50.0 mm | 250 mm | 5.0 μm | 175 Å | On request |
| Luknova | Flex C18-120 | Reversed-Phase | Silica/C18 | 3.0 mm | 30 mm | 3.0 μm | 120 Å | On request |
| Bonna-Agela Technologies | Venusil ASB C18 | Reversed-Phase | Silica/C18 | 30.0 mm | 250 mm | 5.0 μm | 150 Å | On request |
| Thermo Fisher Scientific | BioBasic | Reversed-Phase | Silica/C18 | 0.075 mm | 50 mm | 5.0 μm | — | On request |
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