by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C18 | Pore size | 200 |
| Modifification | Silica/C18 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 18.0 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| RESTEK | Viva | Reversed-Phase | Silica/C18 | 4.6 mm | 100 mm | 5.0 μm | 300 Å | On request |
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/C18 | 1.0 mm | 100 mm | 3.0 μm | 175 Å | On request |
| ES Industries | Chromegabond 1000 MC-18 | Reversed-Phase | Silica/C18 | 4.0 mm | 250 mm | 5.0 μm | 1000 Å | On request |
| Vertical Chromatography | VertiSep GES C18 | Reversed-Phase | Silica/C18 | 10.0 mm | 250 mm | 10.0 μm | 120 Å | On request |
| Sepax Technologies | Sepax HP 100 C18(2) | Reversed-Phase | Silica/C18 | 30.0 mm | 50 mm | 5.0 μm | 100 Å | On request |
| Thermo Fisher Scientific | BetaSil | Reversed-Phase | Silica/C18 | 1.0 mm | 30 mm | 5.0 μm | 100 Å | On request |
| KYA Technologies Corporation | HiQ Sil C18W - 120 | Reversed-Phase | Silica/C18 | 4.0 mm | 100 mm | 3.0 μm | 120 Å | On request |
| GL Sciences | Inertsil ODS-3 | Reversed-Phase | Silica/C18 | 1.5 mm | 50 mm | 4.0 μm | 100 Å | On request |
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