by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C18 | Pore size | 200 |
| Modifification | Silica/C18 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 18.0 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Luknova | Poro C18-200 | Reversed-Phase | Silica/C18 | 4.6 mm | 150 mm | 3.0 μm | 200 Å | On request |
| Princeton Chromatography Inc. | Ultima C18-100 | Reversed-Phase | Silica/C18 | 2.0 mm | 250 mm | 5.0 μm | 100 Å | On request |
| RSTech | HECTOR-W C18 | Reversed-Phase | Silica/C18 | 21.2 mm | 250 mm | 10.0 μm | 300 Å | On request |
| Agilent Technologies | Agilent Polaris | Reversed-Phase | Silica/C18 | 2.0 mm | 75 mm | 3.0 μm | 180 Å | On request |
| GL Sciences | Inertsil ODS-P | Reversed-Phase | Silica/C18 | 4.0 mm | 150 mm | 3.0 μm | 100 Å | On request |
| Imtakt | Cadenza 5CW-C18 | Reversed-Phase | Silica/C18 | 0.5 mm | 150 mm | 5.0 μm | 300 Å | On request |
| Phenomenex | Synergi Fusion-RP | Reversed-Phase | Silica/C18 | 21.0 mm | 250 mm | 4.0 μm | 80 Å | On request |
| Agilent Technologies | Original ZORBAX | Reversed-Phase | Silica/C18 | 9.0 mm | 250 mm | 5.0 μm | 80 Å | On request |
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