by Higgins Analytical
300Å wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120Å) typically used for small molecule analysis. 300Å sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials. Higgins Analytical’s new PROTO™ 200 presents a powerful alternative for the analysis of peptides of ~19kD and less.
| Vendor | Higgins Analytical | Internal Diameter | |
| Brand | PROTO 200 C18 | Pore size | 200 |
| Modifification | Silica/C18 | Surface Area | 200 |
| Mode | Reversed-Phase | Carbon Load, % | 18.0 |
| Particle Size | Endcapped | Yes | |
| Length |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| ACE | ACE C18-HL | Reversed-Phase | Silica/C18 | 4.6 mm | 30 mm | 3.0 μm | 90 Å | On request |
| Agilent Technologies | Agilent Polaris | Reversed-Phase | Silica/C18 | 1.0 mm | 250 mm | 3.0 μm | 180 Å | On request |
| Princeton Chromatography Inc. | Ultima C18-100 | Reversed-Phase | Silica/C18 | 4.6 mm | 250 mm | 5.0 μm | 100 Å | On request |
| Sepax Technologies | Sepax BR 120 C18 | Reversed-Phase | Silica/C18 | 1.0 mm | 150 mm | 3.0 μm | 120 Å | On request |
| BISCHOFF | ProntoSIL 300-3-C18 H | Reversed-Phase | Silica/C18 | 4.0 mm | 250 mm | 3.0 μm | 300 Å | On request |
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/C18 | 4.6 mm | 50 mm | 5.0 μm | 175 Å | On request |
| Sepax Technologies | Sepax HP 120 C18 | Reversed-Phase | Silica/C18 | 10.0 mm | 250 mm | 5.0 μm | 120 Å | On request |
| Imtakt | Unison UK-C18 UP | Reversed-Phase | Silica/C18 | 2.0 mm | 30 mm | 3.0 μm | 130 Å | On request |
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