by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C4 | Surface Area | 100 |
| Modifification | Silica/C4 | Carbon Load, % | 3.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Unimicro Technologies Inc. | Globalsil-120-5-C4 | Reversed-Phase | Silica/C4 | 4.6 mm | 100 mm | 5.0 μm | 120 Å | On request |
| Welch Materials | Ultisil XB-C4 | Reversed-Phase | Silica/C4 | 3.0 mm | 100 mm | 3.0 μm | 300 Å | On request |
| Luknova | HighSelect C4-120 | Reversed-Phase | Silica/C4 | 10.0 mm | 50 mm | 10.0 μm | 120 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/C4 | 2.0 mm | 100 mm | 5.0 μm | 100 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C4-60 | Reversed-Phase | Silica/C4 | 4.6 mm | 250 mm | 3.0 μm | 60 Å | On request |
| Thermo Fisher Scientific | Hypurity | Reversed-Phase | Silica/C4 | 3.0 mm | 50 mm | 5.0 μm | 190 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/C4 | 62.0 mm | 300 mm | 5.0 μm | 100 Å | On request |
| Higgins Analytical | PROTO 200 C4 | Reversed-Phase | Silica/C4 | 10.0 mm | 100 mm | 10.0 μm | 200 Å | On request |
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