by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C4 | Surface Area | 100 |
| Modifification | Silica/C4 | Carbon Load, % | 3.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Princeton Chromatography Inc. | PrincetonSPHER C4-100 | Reversed-Phase | Silica/C4 | 7.8 mm | 50 mm | 10.0 μm | 100 Å | On request |
| Luknova | HighSelect C4-120 | Reversed-Phase | Silica/C4 | 30.0 mm | 250 mm | 10.0 μm | 120 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/C4 | 3.0 mm | 250 mm | 10.0 μm | 100 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C4-100 | Reversed-Phase | Silica/C4 | 4.6 mm | 250 mm | 10.0 μm | 100 Å | On request |
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/C4 | 1.0 mm | 20 mm | 1.9 μm | 175 Å | On request |
| GRACE | Vydac TP C4 | Reversed-Phase | Silica/C4 | 1.0 mm | 150 mm | 5.0 μm | 300 Å | On request |
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/C4 | 1.0 mm | 100 mm | 1.9 μm | 175 Å | On request |
| Higgins Analytical | PROTO 300 C4 | Reversed-Phase | Silica/C4 | 0.5 mm | 30 mm | 5.0 μm | 300 Å | On request |
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