by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C18 | Surface Area | 100 |
| Modifification | Silica/C18 | Carbon Load, % | 8.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| RESTEK | Viva | Reversed-Phase | Silica/C18 | 1.0 mm | 100 mm | 3.0 μm | 300 Å | On request |
| Sepax Technologies | Sepax HP 100 C18(2) | Reversed-Phase | Silica/C18 | 4.6 mm | 50 mm | 5.0 μm | 100 Å | On request |
| Imtakt | Unison UK-C18 UP | Reversed-Phase | Silica/C18 | 2.0 mm | 150 mm | 3.0 μm | 130 Å | On request |
| Dionex | Acclaim PepMap RSLC | Reversed-Phase | Silica/C18 | 0.1 mm | 150 mm | 2.0 μm | 100 Å | On request |
| ES Industries | Chromegabond 100 MC-18 | Reversed-Phase | Silica/C18 | 4.0 mm | 150 mm | 5.0 μm | 100 Å | On request |
| Agilent Technologies | Pursuit XRs | Reversed-Phase | Silica/C18 | 2.0 mm | 150 mm | 5.0 μm | 100 Å | On request |
| GRACE | Vydac Everest C18 | Reversed-Phase | Silica/C18 | 0.3 mm | 250 mm | 5.0 μm | 300 Å | On request |
| SGE Analytical Science | ProteCol C18 H (PEEK Coated) | Reversed-Phase | Silica/C18 | 2.1 mm | 50 mm | 5.0 μm | 120 Å | On request |
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