by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C4 | Surface Area | 100 |
| Modifification | Silica/C4 | Carbon Load, % | 3.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Welch Materials | Ultisil XB-C4 | Reversed-Phase | Silica/C4 | 4.6 mm | 250 mm | 10.0 μm | 300 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/C4 | 62.0 mm | 300 mm | 10.0 μm | 100 Å | On request |
| Sepax Technologies | Sepax Bio 300 C4 | Reversed-Phase | Silica/C4 | 10.0 mm | 250 mm | 10.0 μm | 300 Å | On request |
| Higgins Analytical | PROTO 200 C4 | Reversed-Phase | Silica/C4 | 0.3 mm | 100 mm | 5.0 μm | 200 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/C4 | 4.6 mm | 150 mm | 5.0 μm | 100 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C4-100 | Reversed-Phase | Silica/C4 | 50.0 mm | 100 mm | 10.0 μm | 100 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C4-100 | Reversed-Phase | Silica/C4 | 30.0 mm | 150 mm | 5.0 μm | 100 Å | On request |
| RESTEK | Ultra | Reversed-Phase | Silica/C4 | 3.0 mm | 30 mm | 5.0 μm | 100 Å | On request |
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