by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C18 | Surface Area | 100 |
| Modifification | Silica/C18 | Carbon Load, % | 8.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Thermo Fisher Scientific | BioBasic | Reversed-Phase | Silica/C18 | 2.1 mm | 100 mm | 5.0 μm | 300 Å | On request |
| Higgins Analytical | HEAVY C18 | Reversed-Phase | Silica/C18 | 0.3 mm | 30 mm | 5.0 μm | 100 Å | On request |
| Chromenta | Chromenta BB C18 | Reversed-Phase | Silica/C18 | 4.0 mm | 250 mm | 5.0 μm | 300 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER OMNI C18-100 | Reversed-Phase | Silica/C18 | 4.0 mm | 150 mm | 10.0 μm | 100 Å | On request |
| MACHEREY-NAGEL | NUCLEOSIL C18 | Reversed-Phase | Silica/C18 | 4.6 mm | 125 mm | 5.0 μm | 100 Å | 226.00 |
| SiliCYCLE | SiliaChrom SB C18 | Reversed-Phase | Silica/C18 | 20.0 mm | 250 mm | 5.0 μm | 150 Å | On request |
| GRACE | VisionHT C18 Polar | Reversed-Phase | Silica/C18 | 7.0 mm | 33 mm | 1.5 μm | 100 Å | On request |
| Imtakt | Cadenza HS-C18 | Reversed-Phase | Silica/C18 | 0.2 mm | 150 mm | 3.0 μm | N/A Å | On request |
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