by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C4 | Surface Area | 100 |
| Modifification | Silica/C4 | Carbon Load, % | 3.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Sepax Technologies | Sepax Bio 300 C4 | Reversed-Phase | Silica/C4 | 1.0 mm | 50 mm | 5.0 μm | 300 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C4-300 | Reversed-Phase | Silica/C4 | 30.0 mm | 100 mm | 10.0 μm | 300 Å | On request |
| Princeton Chromatography Inc. | PrincetonSPHER C4-300 | Reversed-Phase | Silica/C4 | 4.0 mm | 100 mm | 5.0 μm | 300 Å | On request |
| Fortis Technologies | FortisBIO C4 | Reversed-Phase | Silica/C4 | 0.075 mm | 150 mm | 5.0 μm | 300 Å | On request |
| RESTEK | Ultra | Reversed-Phase | Silica/C4 | 4.6 mm | 200 mm | 5.0 μm | 100 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/C4 | 8.0 mm | 120 mm | 3.0 μm | 100 Å | On request |
| Welch Materials | Ultisil XB-C4 | Reversed-Phase | Silica/C4 | 3.0 mm | 150 mm | 10.0 μm | 300 Å | On request |
| RESTEK | Ultra | Reversed-Phase | Silica/C4 | 4.6 mm | 250 mm | 5.0 μm | 100 Å | On request |
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