by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C4 | Surface Area | 100 |
| Modifification | Silica/C4 | Carbon Load, % | 3.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| RESTEK | Viva | Reversed-Phase | Silica/C4 | 2.1 mm | 50 mm | 5.0 μm | 300 Å | On request |
| Thermo Fisher Scientific | BioBasic | Reversed-Phase | Silica/C4 | 1.0 mm | 50 mm | — | 300 Å | On request |
| Luknova | Poro C4-300 | Reversed-Phase | Silica/C4 | 21.2 mm | 150 mm | 5.0 μm | 300 Å | On request |
| Orochem | Reliasil C4 90 | Reversed-Phase | Silica/C4 | 3.0 mm | 100 mm | 5.0 μm | 90 Å | On request |
| Higgins Analytical | PROTO 200 C4 | Reversed-Phase | Silica/C4 | 0.3 mm | 100 mm | 5.0 μm | 200 Å | On request |
| Sigma Aldrich | Kromasil C4 | Reversed-Phase | Silica/C4 | 30.0 mm | 250 mm | 10.0 μm | 100 Å | On request |
| Teknokroma | TRACER EXCEL 120 C4 | Reversed-Phase | Silica/C4 | 4.0 mm | 250 mm | 3.0 μm | 120 Å | On request |
| GL Sciences | Inertsil WP300 C4 | Reversed-Phase | Silica/C4 | 3.0 mm | 150 mm | 5.0 μm | 300 Å | On request |
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