by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C4 | Surface Area | 100 |
| Modifification | Silica/C4 | Carbon Load, % | 3.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/C4 | 4.0 mm | 100 mm | 3.0 μm | 175 Å | On request |
| Phenomenex | Jupiter C4 | Reversed-Phase | Silica/C4 | 30.0 mm | 250 mm | 15.0 μm | 300 Å | On request |
| Luknova | Poro C4-300 | Reversed-Phase | Silica/C4 | 4.6 mm | 150 mm | 5.0 μm | 300 Å | On request |
| Sepax Technologies | Sepax GP 120 C4 | Reversed-Phase | Silica/C4 | 0.3 mm | 100 mm | 3.0 μm | 120 Å | On request |
| Aapptec | Spirit Protein | Reversed-Phase | Silica/C4 | 2.1 mm | 30 mm | 5.0 μm | 300 Å | On request |
| ACE | ACE C4-300 | Reversed-Phase | Silica/C4 | 2.1 mm | 20 mm | 3.0 μm | 300 Å | On request |
| RESTEK | Viva | Reversed-Phase | Silica/C4 | 4.6 mm | 200 mm | 5.0 μm | 300 Å | On request |
| Biotage | Resolux 200 C4 | Reversed-Phase | Silica/C4 | 2.1 mm | 150 mm | 4.5 μm | 200 Å | On request |
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