by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C18 | Surface Area | 100 |
| Modifification | Silica/C18 | Carbon Load, % | 8.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/C18 | 3.0 mm | 100 mm | 1.9 μm | 175 Å | On request |
| Teknokroma | Hyperpack ODS | Reversed-Phase | Silica/C18 | 10.0 mm | 150 mm | 5.0 μm | — | On request |
| WATERS | Symmetry C18 | Reversed-Phase | Silica/C18 | 2.1 mm | 150 mm | 3.5 μm | 100 Å | On request |
| Teknokroma | Europa Peptide 120 C18 | Reversed-Phase | Silica/C18 | 4.0 mm | 40 mm | 5.0 μm | 120 Å | On request |
| CHEMINDIA | CHEMSIL-AQUA-C18 | Reversed-Phase | Silica/C18 | 2.0 mm | 100 mm | 3.0 μm | 100 Å | On request |
| Luknova | Poro C18-200 | Reversed-Phase | Silica/C18 | 7.8 mm | 250 mm | 10.0 μm | 200 Å | On request |
| Sepax Technologies | Sepax HP 120 C18 | Reversed-Phase | Silica/C18 | 10.0 mm | 50 mm | 5.0 μm | 120 Å | On request |
| ACE | ACE C18-HL | Reversed-Phase | Silica/C18 | 2.1 mm | 75 mm | 5.0 μm | 90 Å | On request |
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