by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C18 | Surface Area | 100 |
| Modifification | Silica/C18 | Carbon Load, % | 8.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Bonna-Agela Technologies | Venusil XBP C18 | Reversed-Phase | Silica/C18 | 10.0 mm | 150 mm | 10.0 μm | 100 Å | On request |
| Merck | Purospher® STAR | Reversed-Phase | Silica/C18 | 3.0 mm | 125 mm | 3.0 μm | 120 Å | On request |
| Dionex | Acclaim 120 C18 | Reversed-Phase | Silica/C18 | 0.3 mm | 250 mm | 5.0 μm | 120 Å | On request |
| GRACE | Vydac TP C18 | Reversed-Phase | Silica/C18 | 4.6 mm | 150 mm | 5.0 μm | 300 Å | On request |
| BISCHOFF | ProntoSIL 300-3-C18 ace-EPS | Reversed-Phase | Silica/C18 | 4.6 mm | 150 mm | 3.0 μm | 300 Å | On request |
| CHEMINDIA | CHEMSIL-BDS-C18 | Reversed-Phase | Silica/C18 | 4.6 mm | 35 mm | 3.0 μm | 100 Å | On request |
| KNAUER | Eurospher II | Reversed-Phase | Silica/C18 | 4.0 mm | 125 mm | 5.0 μm | 100 Å | On request |
| GRACE | VisionHT C18 Classic | Reversed-Phase | Silica/C18 | 3.0 mm | 150 mm | 5.0 μm | 100 Å | On request |
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