by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C4 | Surface Area | 100 |
| Modifification | Silica/C4 | Carbon Load, % | 3.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| SiliCYCLE | SiliaChrom XDB1 C4 | Reversed-Phase | Silica/C4 | 2.1 mm | 50 mm | 5.0 μm | 100 Å | On request |
| Biotage | Resolux 300 C4 | Reversed-Phase | Silica/C4 | 2.1 mm | 250 mm | 4.5 μm | 300 Å | On request |
| Thermo Fisher Scientific | BioBasic | Reversed-Phase | Silica/C4 | 2.1 mm | 100 mm | — | 300 Å | On request |
| KYA Technologies Corporation | HiQ Sil C4-120 | Reversed-Phase | Silica/C4 | 0.3 mm | 250 mm | 5.0 μm | 120 Å | On request |
| Chromenta | Chromenta EP C4 | Reversed-Phase | Silica/C4 | 2.1 mm | 100 mm | 3.0 μm | 120 Å | On request |
| Higgins Analytical | PROTO 200 C4 | Reversed-Phase | Silica/C4 | 4.0 mm | 30 mm | 5.0 μm | 200 Å | On request |
| Sepax Technologies | Sepax GP 120 C4 | Reversed-Phase | Silica/C4 | 1.0 mm | 150 mm | 4.0 μm | 120 Å | On request |
| ACE | ACE C4-300 | Reversed-Phase | Silica/C4 | 21.2 mm | 150 mm | 10.0 μm | 300 Å | On request |
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