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SH1-100.10.0530

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SH1-100.10.0530
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SHARC 300

by SIELC

SHARC™ HPLC columns are the latest innovation from SIELC Technologies, the inventors of Primesep®. SHARC columns are the first commercially available columns with separation based primarily on hydrogen bonding. SHARC stands for  Specific Hydrogen-bond Adsorption Resolution Chromatography.

Hydrogen bonding is an interaction between hydrogen atom bound to electronegative atoms in a molecule, such as oxygen, nitrogen, fluorine. This is typically a weak interaction, especially when separation is performed in aqueous solutions. Liquid chromatography techniques evolved as tool for separation of different molecules based on their physico-chemical properties. Most common techniques of the separation are:

  • Hydrophobic separation based on degree of hydrophobicity of the molecule
  • Ion-exchange separation based on number, nature, and distribution of charges in the molecule.
  • Normal phase separation based on hydrophilic properties of the molecules including molecule dipoles value and dipoles position
  • Size exclusion separation (SEC) based on molecule size and shape
Application areas:
Broad range of analytes Geometrical isomers Mixtures with differing retention Positional isomers
Specification
Vendor SIELC Particle Size
Brand SHARC 300 Length
Modifification Hydrogen bond Internal Diameter
Mode Normal-Phase/Reversed-Phase/HIC/Size-Exclusion Pore size 300
Similar columns Columns with same mode & modification — across all brands
VendorBrandModeModification Int. diam.LengthParticlesPoresPrice
SIELC SHARC 100 Normal-Phase/Reversed-Phase/HIC/Size-Exclusion Hydrogen bond 10.0 mm 50 mm 5.0 μm 100 Å On request
SIELC SHARC 300 Normal-Phase/Reversed-Phase/HIC/Size-Exclusion Hydrogen bond 3.2 mm 25 mm 5.0 μm 300 Å On request
SIELC SHARC 300 Normal-Phase/Reversed-Phase/HIC/Size-Exclusion Hydrogen bond 4.6 mm 150 mm 3.0 μm 300 Å On request
SIELC SHARC 300 Normal-Phase/Reversed-Phase/HIC/Size-Exclusion Hydrogen bond 1.0 mm 10 mm 10.0 μm 300 Å On request
SIELC SHARC 300 Normal-Phase/Reversed-Phase/HIC/Size-Exclusion Hydrogen bond 3.2 mm 250 mm 3.0 μm 300 Å On request
SIELC SHARC 100 Normal-Phase/Reversed-Phase/HIC/Size-Exclusion Hydrogen bond 1.0 mm 100 mm 3.0 μm 100 Å On request
SIELC SHARC 300 Normal-Phase/Reversed-Phase/HIC/Size-Exclusion Hydrogen bond 2.1 mm 100 mm 3.0 μm 300 Å On request
SIELC SHARC 300 Normal-Phase/Reversed-Phase/HIC/Size-Exclusion Hydrogen bond 1.0 mm 250 mm 3.0 μm 300 Å On request
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