050.17HY0502

PolyHYDROXYETHYL A - for Hydrophilic Interaction and Size Exclusion HPLC

This neutral, polar material was developed specifically for Hydrophilic Interaction Chromatography (HILIC). HILIC is a variant of normal phase chromatography that is performed with a polar stationary phase and a partly aqueous mobile phase. This permits normal-phase separations of peptides, nucleic acids, carbohydrates, some proteins, and polar solutes in general. In comparison with other materials used for HILIC, PolyHYDROXYETHYL A affords sharper peaks and better selectivity and recovery. Its great polarity means less organic solvent is needed to get retention.

Use PolyHYDROXYETHYL A for:

• Analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules (amino acids; methotrexate) in plasma or crude extracts.
• Peptide separations and mapping involving differences in polar groups (Ser-; glycopeptides; etc.).
• Multidimensional purification of synthetic and natural peptides or fractionation of really complex digests.
• HPLC of solutes that aren't soluble in aqueous media (membrane proteins;phospholipids).
• Eliminating detergents, lipids, and salts from a sample.
• Oligonucleotides and their analogs.