by Orochem
Affinity Chromatography is a very powerful technique commonly used to purify a biomolecule of interest based on the specific interaction of the biomolecule with a ligand immobilized on a stationary phase. Affinity chromatography is widely used in biotechnology research and in industry. Using this technique, molecules of interest can be purified in a one-step separation with high purity and high recovery.
Immobilized metal chelate chromatography (IMAC) is a widely used method for purification of recombinant His-tagged proteins. Nickel-NTA resin is packed into HPLC column for multiple rounds of affinity purification.
| Vendor | Orochem | Mode | IMAC |
| Brand | EZPure Ni-NTA | Length | 50 |
| Modifification | Nickel-NTA resin | Internal Diameter | 4.6 |
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