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63665

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ProPac HIC-10

by Dionex

 

Hydrophobic Interaction Chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.

  • Based on 5 µm ultrahigh purity spherical silica gel particles, with 300 Å pores
  • Proteins separated under non-denaturing conditions
  • High protein loading capacity for protein purification applications
  • Proprietary multidentate silica bonding for enhanced hydrolytic stability
  • High-resolution HPLC separation of proteins and peptides
  • Wide range of applications

The ProPac® HIC-10 column is a high-resolution, high-capacity, silica-based HIC column that provides greater hydrolytic stability under the highly aqueous conditions used in HIC. Examples include the separation of bovine serum proteins, snake venom proteins, enzymes, human skeletal muscle protein (HSMP), monoclonal antibodies, pancreatin, and thrombin and peptide applications including tryptic digests of proteins.

Application areas:
Antibody Bio-molecules Large molecules Peptides Proteins
Specification
Vendor Dionex Internal Diameter 7.8
Brand ProPac HIC-10 Pore size 300
Mode HIC pH stability from 2.5
Particle Size 5.0 pH stability to 7.5
Length 75
Similar columns Based on mode & modification
VendorBrandModeModification Int. diam.LengthParticlesPoresPrice
Dionex ProPac HIC-10 HIC — 2.1 mm 100 mm 5.0 μm 300 Å On request
Dionex ProPac HIC-10 HIC — 4.6 mm 100 mm 5.0 μm 300 Å On request
Dionex ProPac HIC-10 HIC — 4.6 mm 250 mm 5.0 μm 300 Å On request
TOSOH TSKgel HIC Polymethacrylate/ Butyl 4.6 mm 35 mm 2.5 μm — On request
TOSOH TSKgel HIC Polymethacrylate/ Butyl 4.6 mm 100 mm 2.5 μm — On request
TOSOH TSKgel HIC Polymethacrylate/ Ether 8.0 mm 75 mm 10.0 μm 1000 Å On request
TOSOH TSKgel HIC Polymethacrylate/ Ether 2.0 mm 75 mm 10.0 μm 1000 Å On request
TOSOH TSKgel HIC Polymethacrylate/ Ether 7.5 mm 75 mm 10.0 μm 1000 Å On request
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