by MACHEREY-NAGEL
NUCLEOSIL® standard silica phases for HPLC
Features
NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure
NUCLEOSIL® C8 HD - Octyl modified high density silica phase for HPLC
Monomeric modification, endcapped · 13 % C · USP L7
Features
Hydrophobic, nonpolar phase for RP chromatography, pore size 100 Å, particle sizes 5 µm
pH stability 2–8, carbon content 13 %,
recommended for separation of moderately to highly polar (water-soluble) compounds
Applications: steroids, nucleosides, cyclodextrins, pharmacological plant constituents
Separation mechanism based on hydrophobic (van der Waals) interactions
| Vendor | MACHEREY-NAGEL | Max Pressure | 5800 |
| Brand | NUCLEOSIL C8 HD | Surface Area | 350 |
| Modifification | Silica/C8 | Carbon Load, % | 20.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | Operating Temperature | 60 | |
| Length | pH stability from | 2.0 | |
| Internal Diameter | pH stability to | 9.0 | |
| Pore size | 100 |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Sigma Aldrich | Kromasil C8 | Reversed-Phase | Silica/C8 | 4.6 mm | 150 mm | 13.0 μm | 100 Å | On request |
| Vertical Chromatography | VertiSep EPS C8 | Reversed-Phase | Silica/C8 | 21.2 mm | 250 mm | 5.0 μm | 100 Å | On request |
| Greyhound | GenSphere C8 300 | Reversed-Phase | Silica/C8 | 4.0 mm | 150 mm | 5.0 μm | 300 Å | On request |
| CERI | L-column2 C8 | Reversed-Phase | Silica/C8 | 4.6 mm | 50 mm | 5.0 μm | 120 Å | On request |
| Teknokroma | Hyperpack C8 | Reversed-Phase | Silica/C8 | 4.0 mm | 150 mm | 5.0 μm | — | On request |
| GL Sciences | Inertsil C8-3 | Reversed-Phase | Silica/C8 | 1.0 mm | 50 mm | 5.0 μm | 100 Å | On request |
| Luknova | HighSelect C8-120 | Reversed-Phase | Silica/C8 | 10.0 mm | 100 mm | 5.0 μm | 120 Å | On request |
| Welch Materials | Topsil C8 | Reversed-Phase | Silica/C8 | 4.6 mm | 30 mm | 3.0 μm | 150 Å | On request |
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