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720901.4
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NUCLEOSIL C4

by MACHEREY-NAGEL

NUCLEOSIL® standard silica phases for HPLC

Features

NUCLEOSIL® is a family of totally porous spherical silicas with a very pure and uniform SiO2 structure

  • Wide acceptance as routine packings for very different fields of chromatography
  • One of the first spherical silicas used in HPLC
  • Developed in the early seventies, it became a world-renowned HPLC packing
  • Still found in many analytical and preparative applications
  • An absolutely reliable choice in HPLC
  • The largest variety of modified HPLC silicas available on the market
     

NUCLEOSIL® C4 - Butyl modified silica phases for HPLC
Medium density modification, endcapping · ~ 2 % C · USP L26

Features

Endcapped phases for RP and ion-pairing chromatography
Available pore sizes 120, 300, 500, 1000 and 4000 Å, available particle sizes 5 µm, 7 µm and 10 µm
Separation mechanism based on hydrophobic (van der Waals) interactions, some residual silanol interactions
Retention times are shorter than on C8 and C18 phases
pH stability at 20 °C: 2–8

Application areas:
Hydrophobic compounds Peptides Proteins
Specification
Vendor MACHEREY-NAGEL Max Pressure 4350
Brand NUCLEOSIL C4 Surface Area 100
Modifification Silica/C4 Carbon Load, % 2.0
Mode Reversed-Phase Endcapped Yes
Particle Size Operating Temperature 60
Length pH stability from 2.0
Internal Diameter pH stability to 8.0
Pore size 300
Similar columns Columns with same mode & modification — across all brands
VendorBrandModeModification Int. diam.LengthParticlesPoresPrice
Higgins Analytical PROTO 300 C4 Reversed-Phase Silica/C4 0.5 mm 50 mm 5.0 μm 300 Å On request
ACE ACE C4-300 Reversed-Phase Silica/C4 0.5 mm 50 mm 5.0 μm 300 Å On request
Orochem Reliasil C4 90 Reversed-Phase Silica/C4 50.0 mm 250 mm 10.0 μm 90 Å On request
KNAUER Eurosil Bioselect Reversed-Phase Silica/C4 4.0 mm 300 mm 10.0 μm 300 Å On request
Aapptec Spirit Protein Reversed-Phase Silica/C4 4.0 mm 100 mm 5.0 μm 300 Å On request
Dikma Technologies Inc. Bio-Bond C4 Reversed-Phase Silica/C4 4.6 mm 250 mm 5.0 μm 300 Å 606.00
KNAUER Eurospher II Reversed-Phase Silica/C4 2.0 mm 125 mm 10.0 μm 100 Å On request
SGE Analytical Science ProteCol C4 H (PEEK Coated) Reversed-Phase Silica/C4 3.0 mm 50 mm 3.0 μm 300 Å On request
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