by Higgins Analytical
Low pH operating conditions and high pH column washing steps are typical bioprocessing operating conditions that can severely limit the lifetime and performance of widepore columns typically used for protein and peptide analysis. Higgins Analytical’s new PROTO300 has high capacity for enhanced resolution and is robust enough to withstand prolonged use at extreme pH (1.5 - 10).
| Vendor | Higgins Analytical | Pore size | 300 |
| Brand | PROTO 300 C18 | Surface Area | 100 |
| Modifification | Silica/C18 | Carbon Load, % | 8.0 |
| Mode | Reversed-Phase | Endcapped | Yes |
| Particle Size | pH stability from | 1.5 | |
| Length | pH stability to | 10.0 | |
| Internal Diameter |
| Vendor | Brand | Mode | Modification | Int. diam. | Length | Particles | Pores | Price |
|---|---|---|---|---|---|---|---|---|
| Sepax Technologies | Sepax Bio 300 C18 | Reversed-Phase | Silica/C18 | 0.75 mm | 50 mm | 3.0 μm | 300 Å | On request |
| Thermo Fisher Scientific | BioBasic | Reversed-Phase | Silica/C18 | 2.1 mm | 150 mm | 5.0 μm | 300 Å | On request |
| Thermo Fisher Scientific | Hypesil GOLD | Reversed-Phase | Silica/C18 | 3.0 mm | 100 mm | 3.0 μm | 175 Å | On request |
| Thermo Fisher Scientific | BioBasic | Reversed-Phase | Silica/C18 | 0.075 mm | 50 mm | 5.0 μm | — | On request |
| Agilent Technologies | Original ZORBAX | Reversed-Phase | Silica/C18 | 4.0 mm | 250 mm | 5.0 μm | 80 Å | On request |
| MACHEREY-NAGEL | NUCLEODUR C18 ISIS | Reversed-Phase | Silica/C18 | 4.6 mm | 50 mm | 1.8 μm | 110 Å | 479.00 |
| Dikma Technologies Inc. | Spursil C18 | Reversed-Phase | Silica/C18 | 2.1 mm | 150 mm | 5.0 μm | 100 Å | 577.00 |
| Vertical Chromatography | VertiSep AQS C18 | Reversed-Phase | Silica/C18 | 10.0 mm | 150 mm | 5.0 μm | 120 Å | On request |
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